Wolf-like or dog-like? A comparison of gazing behaviour across three dog breeds tested in their familiar environments

Human-directed gazing, a keystone in dog\u2013human communication, has been suggested to derive from both domestication and breed selection. The influence of genetic similarity to wolves and selective pressures on human-directed gazing is still under debate. Here, we used the \u2018unsolvable task\u2019 to compare Czechoslovakian Wolfdogs (CWDs, a close-to-wolf breed), German Shepherd Dogs (GSDs) and Labrador Retrievers (LRs). In the \u2018solvable task\u2019, all dogs learned to obtain the reward; however, differently from GSDs and LRs, CWDs rarely gazed at humans. In the \u2018unsolvable task\u2019, CWDs gazed significantly less towards humans compared to LRs but not to GSDs. Although all dogs were similarly motivated to explore the apparatus, CWDs and GSDs spent a larger amount of time in manipulating it compared to LRs. A clear difference emerged in gazing at the experimenter versus owner. CWDs gazed preferentially towards the experimenter (the unfamiliar subject manipulating the food), GSDs towards their owners and LRs gazed at humans independently from their level of familiarity. In conclusion, it emerges that the artificial selection operated on CWDs produced a breed more similar to ancient breeds (more wolf-like due to a less-intense artificial selection) and not very human-oriented. The next step is to clarify GSDs\u2019 behaviour and better understand the genetic role of this breed in shaping CWDs\u2019 heterospecific behaviour

Ecological relevance of Sentinels' biomarker responses: A multi-level approach

In response to the need for more sensitive and rapid indicators of environmental quality, sublethal effects on the lowest levels of biological organization have been investigated. The ecological relevance of these responses assumes a prevailing role to assure effectiveness as indicator of ecological status. This study aimed to investigate the linkages between biomarker responses of caged bivalves and descriptive parameters of macrobenthic community structure. For this purpose a multi-level environmental assessment of marine and estuarine zones was performed in São Paulo coast, Brazil. Multivariate analysis was applied to identify linkages between biological responses and ecological indices, as well as to characterizing the studied stations. Individuals of the marine mussel Perna perna caged along Santos Bay showed signs of oxidative stress, lysosomal membrane destabilization, histological alterations and reduced embryonic development. The estuarine oyster Crassostrea rhizophorae caged along Santos Port Channel showed alterations on biotransformation enzymes and antioxidant system, DNA damage and lysosomal membrane destabilization. The benthic community analysis showed reduced richness and diversity in the same areas of the Santos bay and estuary where biomarker responses were altered. Our results revealed that xenobiotics are inducing physiological stress, which may lead to changes of the benthic community structure and deterioration of the ecological status over time. Integrating biomarker responses and ecological indexes improved certainty that alterations found at community level could be related to xenobiotic as stressors, which was very useful to improve the discriminatory power of the environmental assessment.CAPES/MECD-DGU # 099/06CAPES- BEX2492/08-03CAPES - BEX2300/08-7FAPESP - 98/00808-6CNPq - 308649/2011-7CAPES PAEX 1155-13-